EPA Method 

Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms.

• Pimephales promelas = fathead minnow, EPA 2000.0
• Ceriodaphnia dubia = daphnia, EPA 2002.0   

Sample Collection Requirements for WET Testing

• For toxicity testing only temperature preservation is permitted, refrigeration or icing immediately upon collection is required. Samples must be received at <6 degrees Celsius.
• A Chain of Custody (COC) form with the date and time sampling occurred is required for WET samples.
• The maximum hold time for WET testing samples is 36 hours. The test must be set before the 36 hour time period expires.                                                                                                                                                                                 

Acute WET Testing Conditions

• Duration is 48 hours for C.dubia and 48-96 hours for P.promelas
• Tests are conducted at 25±1°C (environmental chamber)
• Typically receiving water is used for dilution water, however laboratory water can be used if none is available
• Test Concentrations: 5 for a definitive, 1 (100%) for Screens
• Test Replicates: Typically 4 per concentration for C.dubia and 2 per concentration for P. promelas
• Number or organisms per concentration: Typically 20 per concentration for C.dubia and P.promelas
• Age of test organisms: <24 hours for C.dubia and 1-14 days old for the P.promelas

For More Information:

Please contact Chad Cooper at [email protected] or call 1-417-864 -8924.