Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms.
- Pimephales promelas = fathead minnow, EPA 2000.0
- Ceriodaphnia dubia = daphnia, EPA 2002.0
Sample Collection Requirements for WET Testing
- For toxicity testing only temperature preservation is permitted, refrigeration or icing immediately upon collection is required. Samples must be received at <6 degrees Celsius.
- A Chain of Custody (COC) form with the date and time sampling occurred is required for WET samples.
- The maximum hold time for WET testing samples is 36 hours. The test must be set before the 36 hour time period expires.
Acute WET Testing Conditions
- Duration is 48 hours for C.dubia and 48-96 hours for P.promelas
- Tests are conducted at 25±1°C (environmental chamber)
- Typically receiving water is used for dilution water, however laboratory water can be used if none is available
- Test Concentrations: 5 for a definitive, 1 (100%) for Screens
- Test Replicates: Typically 4 per concentration for C.dubia and 2 per concentration for P. promelas
- Number or organisms per concentration: Typically 20 per concentration for C.dubia and P.promelas
- Age of test organisms: <24 hours for C.dubia and 1-14 days old for the P.promelas
For More Information:
Please contact Chad Cooper at [email protected] or call 1-417-864 -8924.